New registration of three bacterial strains isolated from water samples in Al-Qurna/Basrah Governorate

et al Hassan

Authors

Hala F. Hassan Department of Marine Biology, Marine Science Centre, University of Basrah

Keywords:

New registration, Three isolates, 16s rRNA, Bacteria, Qurna provenance.

Abstract

The purpose of the current work was the isolation and identification of bacterial strains that may be associated with disease and pollution of water. Three sites around Al-Qurna Hospital were selected to collect water samples monthly from March 2026 to May 2026. Samples were frequently taken between 9 a.m. and 12 p.m. Water samples were collected from the surface at a depth of 30 to 40 cm in autoclaved amber bottles. The bottles were pre-washed twice with water samples before being filled. The findings suggested the isolation of three types of isolates namely Enterobacter bugandensis, Enterobacter asburiae and Enterobacter kobei with the percentages 38.2%, 31.7% and 30.1% respectively. In the genetic analysis, the 16S rRNA gene was investigated using PCR approach. A single band with a molecular weight of 1500 bp was used to determine the presence of a gene.

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