Comparative Study of Diets of Mixed-species-zooplankton and Formulated-feed Effects on Physicochemical Parameters, Growth and Survival Rate of Clarias gariepinus Larvae

Austeen  Obhahie Obhahie

Authors

Obhahie et al., 2025 1Dept. of Animal and Environmental Biology (AEB), Fed. Univ. Oye-Ekiti (FUOYE), Nigeria, Dept. of Biological Sciences, National Open University of Nigeria, Headquarters, Jabi, Abuja, Department of AEB, Delta State University (DELSU), Abraka, Nigeria

Keywords:

Clarias gariepinus larvae, Formulated-feed, Live-mixed-species-zooplankton, Physicochemical-parameters, Survival-growth-rate.

Abstract

Comparative analysis of live-mixed-species-zooplankton-diet (LMSZD) and formulated-feed-diet (FFD) effects on culture-media physicochemical parameters, growth and survival rate of Clarias gariepinus larvae were assessed for 15 days. The experiment was conducted in six plastic-aquaria (30 liters) in triplicates, designated as Tanks-A and Tanks-B. Each tank was stocked with 200 individuals 3-days old post-yolk larvae of initial mean-weight and mean-length of 0.0128 g and 0.65 cm respectively. Live-zooplankton-fed-larvae (LZFL) were placed in Tanks-A, while formulated-feed-fed-larvae (FFFL) were in Tanks-B. The larvae were fed twice daily at ad-libitum. Physicochemical parameters analysis was done at two days interval. The cumulative-mean-values recorded in Tanks-A and Tanks-B respectively were; temperature (24.24; 23.88 °C), pH (7.29; 6.96), electrical-conductivity (263.84; 276.7 µs/cm); total-alkalinity (74.99; 93.45 ppm), total-dissolved-solids (127.79; 138.8 ppm), dissolved-oxygen (7.02; 5.08 ppm), biological-oxygen-demand (1.08; 1.77 ppm) and free-carbon-dioxide (239.14; 11.08 ppm). The growth parameters for LZFL and FFFL respectively were length increment (1.20; 1.00 cm), weight gain (0.1755; 0.1456 g) and specific growth rate [SGR] (%) were 17.92; 16.78. The survival rates (%) were 89.50; 55.00, while the Condition Factor [K] (%) were 2.77; 3.53 for LZFL and FFFL respectively. BOD, Temperature, pH, and CO₂ did not have significant difference (P>0.05). EC, TA, TDS, and DO values exhibited significant differences at (P≤0.05). Growth parameters mean exhibited a gradual daily upsurge in both treatments. LZFL maintained a higher mean values than FFFL, through the experiments. Both LZFL and FFFL growth indices values exhibited significant difference (P≤0.05).

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References

Adeyemo, A. A.; Oladosu, G. A. and Ayinla, A. O. (1994). Growth and survival of fry of African catfish species, Clarias gariepinus Burchell, Heterobranchus biodorsalis Geoffrey and Heteroclarias reared on Moina dubia in comparison with other feed sources. Aquaculture, 119: 41 - 45. Elsevier Science B. V., Amsterdam. URL:https//doi.org/10.1016/0044-8486(94)90442-1

Agbebi, O. T.; Faseluka, O. and Idowu, A. A. (2013). Effects of various latency periods on the fertilization, hatchability and survival of Clarias gariepinus. J. fish. & Aquat. Sci. 8(1): 178 – 183. ISSN 1816-4927. DOI:10.3923/jfas.2023.178. 183

Ajah, P. O. (2010). Mass culture of Rotifera (Brachionus quadridentatus [Hermann, 1783], using three different algal species. Afr. J. Food Sci., 4(3) Pp. 80 - 85. https://doi.org/10.5897/AJFS.9000181

Al-Sulivany, B. S. A; Owais, M.; Dernekbaş, S.; Fazal, R.M.; Selamoglu, Z and Shahid, A. (2025). Salinity and Organic Feed Optimization for Artemia franciscana Culture: Differential Survival Responses to Chlorella vulgaris and Saccharo-myces cerevisiae in Nauplii and Adults. Iraqi J. Aquacult. 22(1): 95 - 110. URL: http://ijaqua.uobasrah.edu.iq/index.php/jaqua/article/view/678

Amali, E. I. and Solomon, S. G. (2001). Growth and survival of first feeding larvae of C. gariepinus fed live and preserved zooplankton. J. Aquat. Sci., 16: 29 - 32.

APHA – American Public Health Association; AWWA – Amer. Water Works Assoc. and WPCF – Water Pollut. Control Fed’n. (2017). Std. Methods for the Exam. of Water and Wastewater 23rd Ed., APHA, AWWA, WPCF, New York. URL: www.aphabooksstore.org; www. standardmethods.org - ISSN 55-1979

Arimoro, F. O. (2007). First feeding in the African catfish Clarias anguillaris fry in tanks with the freshwater rotifer Brachionus calyciflorus cultured in a continuous feedback mechanism in comparison with a mixed zooplankton diet. J. Fish. Aquat. Sci., 2(4): 275-284. DOI:10.3923/jfas.2007.275.284 URL: https://scialert.net/abstract/?doi=jfas.2007.275.284

Arimoro, F. O.; Ofojekwu, P. C. and Agbon, A. O. (2008). Growth and survival of the toothed carp, Fundulopanchax gardneri larvae reared in replicate plastic enclosures with different live starter diets. Killi Kontakt, 36 (2): 29 – 41.

Azionu, B. C.; Ovie, S. I. and Adigun, B. (2001). Successional pattern of the plankton community of the integrated poultry-fish reservoir and the adjoining newly constructed reservoir in NIFFR (Natl. Inst. Freshwater Res.), Estate, New Bussa, Nigr. In: NIFFR Annual Report (2000). 9 - 12 Pp. ISSN 0331 - 9296

Boyd, C.E. (1982). Water quality management for pond fish culture. Elseiver Publishers, pp.249.https://www.cabidigitallibrary.org/doi/full/10.5555/ 19821428342

Dhert, P. (1996). Rotifers - Biology and life history, P. 50. In: Manual on the production and the use of live food for aquaculture. Laven, P. and Sorgeloos, P. (eds). FAO Fisheries Technical Paper No. 361, Rome. 295 Pp. URL: http://aquacultura.org/upload/files/pdf/library/fao/Manual%20on%20the%20Production%20and%20Use%20of%20Live%20Food%20for%20Aquaculture.pdf

Eilertsen, M.; Davies, W. I.L.; Patel, D.; Barnes, J. E.; Karlsen, R.; Mountford, J. K.; Stenkamp, D. L.; Patel, J. S. and Helvik, J. V. (2022). An EvoDevo Study of Salmonid Visual Opsin Dynamics and Photopigment Spectral Sensitivity. Front. Neuroanat. 16:945344. doi: 10.3389/fnana.2022.945344

El-Naggar, G.O.; Ibrahim, N. A. and Abou-Zead, M. Y. (2008). Influence of fertilizers’ types and stocking density on water quality and growth performance of Nile Tilapia – African Catfish in polyculture system. 8th International Symposium on Tilapia in Aquacult. WorldFish Center, Regional Center for Africa and West Asia, Abbassa, Abou-Hammad, Sharkia, Egypt. E-mail: g.naggar@cgiarorg URL: https://cales.arizona.edu/azaqua/ista/ISTA8/NabilIbrahem.pdf

Evans, B. I. and Browman, H. I. (2004). Variation in the development of the fish retina. American Fisheries Society Symposium 40:145 - 166. URL:https:/ /www.researchgate.net/publication/232708894_Variation_in_the_development_of_the_fish_retina

Eya, J.C. (2003). Zooplankton production/water quality management. In: Farmer-to- Farmer Program; Manual prepared for Winrock Intl. Funded by the U. S. Agency for International Development.

Fermin, A.C. and Bolivar, M. E. C. (1994). Feeding live or frozen Moina macrocopa (Strauss) to Asian seabass, Lates calcarifer (Bloch), larvae. Israeli Journal of Aquaculture-Bamidgeh, 46(3): 132 – 139. http://hdl.handle.net/10862/1455

Francis-Floyd, R. (2003). Dissolved Oxygen for Fish Production. Fact Sheet FA-27. A serial publication of the Dept of Fish. Aqua. Sci., Florida Coop. Ext. Ser., Inst. Food and Agric. Sci., University of Florida. URL: http://edis.ifas.ufl.edu. 3 Pp.

Gao, N. (2024). Condition Factor for Salmonid Aquaculture. Factsheet. #24-033. Ontario Ministry of Agriculture, Food and Agribusiness (OMAFA). © King’s Printer for Ontario, ISSN 1198-712X. Pp 4. URL: https://www.ontario.ca/files /2024-11/omafa-condition-factor-for-salmonids-en-2024-11-26.pdf

Goswami, S.C. (2004). Zooplankton methodology, collection and identification - a field manual. In: Dhargalkar, V. K. and Verlecar, X.N., (Ed.). Natl. Inst. of Oceanogr., Dona Paula, Goa. Fin. Support, Min. Envir. & Forests, New Delhi. URL:https://drs.nio.res.in/drs/bitstream/handle/2264/95/Zooplankton_ Manual.pdf?sequence=1&is

Hubbs, C. L. and Lagler, K. F. (1964). Fishes of the Great Lakes Region. University of Michigan Press, Ann Arbor, MI, USA, 213 Pp.

Huisman, E. A. (1976). Hatchery and Nursery operation of fish culture management. In: Huisman et. al., (eds.) Aspects of Fish culture and Fish Breeding. Misc. paper 13. Veeman and zone, Wageningen, The Netherlands. 29 - 50 Pp. URL: https://edepot.wur.nl/449468

Huong, D. T. T.; Phuong, N. T. and Ha, N. T. K. (2024). Effects of carbon dioxide (CO2) at different temperatures on embryonic development of striped catfish (Pangasianodon hypophthalmus). AACL Bioflux 17(6): 2549 - 2556. URL: https://bioflux.com.ro/docs/2024.2549-2556.pdf

Janssen, J. A. L. (1987). Mass production of fry and fingerlings of the African catfish Clarias gariepinus. In: Delince, G. A.; Campbell, D.; Janssen, J. A. L. and Kutty, M. N. (eds.). Seed production. ARAC Snr. Aquaculturists Course. ARAC, P/H., Nigeria. UNDP-FAO-UN-NIOMR Project RAF/82/009 July, 1987. URL: https://www.fao.org/4/ac182e/AC182E00.htm#TOC

Kahami, A. A.; Abdullah, A. F. and Al-Shawi, I. J. (2023) A Comparative Study on Water Quality Assessment of Shatt Al-Arab Estuary and Al-Ashar Canal, Basrah, Iraq. Iraqi J. Aquacult. 20(2): 167 - 192. https://doi.org/10.58629/ ijaq.v20i2.484

Lavens, P. and Sorgeloos, P. (1996). Manual on the production and the use of live food for aquaculture. FAO Fisheries Technical Paper No. 361, Rome. 295 pp. URL:http://aquacultura.org/upload/files/pdf/library/fao/Manual%20on%20the%20Production%20and%20Use%20of%20Live%20Food%20for%20Aquaculture.pdf

Madu C. T.; Ufodike, E. B.C and Ita, E. O. (1990). Food and feeding habits of hatching of mudfish, Clarias anguillaris. J. Aquati. Sci. 5:27-31. URL: https://scispace .com/papers/food-and-feeding-habits-of-hatchlings-of-the-mudfish-clarias-5fc3633qs3

Marinou, K.; Villalba, M. T.; Fabris, A.; Henriksen, N. H.; Papaharisis, L.; Pelekanakis, Y.; Pavlidis, M and Hans van de Vis. (2020). Guidelines on water quality and handling for the welfare of farmed vertebrate fish. Official Journal of the European Union C 31). URL: https://food.ec.europa.eu/system/files /2022-07/aw_platform_plat-conc_guide_farmed-fish_en.pdf

Obhahie, A. I.; Ugwaka, K. A.; Ugwu, L. L. and Adesiyan, F. A. (2007). Effects of Industrial Effluents and Municipal Wastes on Water Conductivity and Total Dissolved Solids, Sulphate and Phosphate Ions Concentration of Ogba River, Benin City, Nigeria. J. Fish. Intl. 2(4): 277 - 283. URL: https://www.Makhill publications.co/view-article/1817-3381/jfish.2007.277.283

Obhahie, A. I. (2022). Fish fry nutrition, In: Akintomide, T. O. (ed.). Aquafeed production, economics and health impact in fish management. OAK Ventures publ., Nigr. ISBN 978-978-51961-5-3. 2: 23 - 37. URL: https://vetbooks.ir/ aquafeed-production-economics-and-health-impact-on-fish-management/

Obhahie, A. I.; Oyewumi, J. O. and Agbon, A. O. (2025). Comparative analysis of organic and inorganic fertilizers effects on zooplankton population dynamics and physicochemical parameters of culture-media. Sahel J. Life Sci. FUDMA, 3(2): 211-221s. DOI: https://doi.org/10.33003/sajols-2025-030222-26

Okogwu, O. I.; Obhahie, A. I.; Hamzat, M. and Ovie S. I. (2006). Growth and survival of Heterobranchus bidorsalis and a hybrid catfish, Clarias anguillaris X H. bidorsalis larvae reared on live Moina micrura. Scientia Afr. 5(2):127 - 132.

Okoro, B. C.; Oyeniyi, O. V.; Soboyejo, I. O. and Nwameka, P. C. (2021). Effect of various latency period on fertilization, hatchability and survival of fry of Clarias gariepinus in a partial flow through system. Intl. J. Fish. and Aquat. Res. 6(2): 66 – 68. www.fishjournals.com, ISSN: 2456-7248 URL: https:// www.fishjournals.com/assets/archives/2021/vol6issue2/6-2-14-493.pdf

Okunsebor, S. A. (2014). Culture of Zooplankton (Brachionus calyciflorus, Moina micrura and Daphnia pulex), as Live Food for Heterobranchus bidorsalis Hatchlings. Ph.D. Thesis, Dept. Zool., Univ. Jos, Nigeria: 189 Pp. URL: https:/ /www.semanticscholar.org/paper/Culture-of-Zooplankton-(Brachionus-Calyciflorus%2C-As-Okunsebor/2341bdf8a678569cf6f5f1ed1f6eaee446d3e87e

Olurin, K. B. and Oluwo, A. B. (2010). Growth and Survival of African Catfish (Clarias gariepinus) Larvae Fed Decapsulated Artemia, Live Daphnia or Commercial Starter Diet. The Israeli J. Aquacult. – Bamidgeh. 62(1): 50 - 55. URL: https:/ /scispace.com/pdf/growth-and-survival-of-african-catfish-clarias-gariepinus-2thxt15d27.pdf

Ovie, S. I.; Adepoju, F. and Adigun, B. (1997). Growth survival and nutritive value of post larvae of Heterobranchus longifis fed Moina sp. and mixed zooplankton. In: NIFFR Annual Report (1997). 1 - 6 Pp. ISSN 0331 - 9296

Ovie, S. I.; Adepoju, F. and Ajayi, O. (2001). Limnological stock assessment, productivity and potential fish yield of Dadin Kowa and Kiri reservoirs. In: NIFFR Annual Report (2000). 9 - 12 Pp. ISSN 0331 - 9296

Ovie, S.I. and Ovie, S.O. (2002). Fish-larval rearing: The effect of pure/mixed zooplankton and artificial diet on the growth and survival of Clarias Anguillaris larvae. J. Aquat. Sci. 17(1): 69 - 73. DOI:10.4314/jas.v17i1.19915

Ovie, S.I. and Egborge, A.B.M. (2005). Comparative growth of the larvae of Clarias anguillaris reared on Artemia and live and frozen Moina micrura. J. Aquacult. Trop., 20(3): 233-239.

Pandey, G. (2013). Feed formulation and feeding technology for fishes. Intl. Res. J. Pharm., 4(3):23-30 ISSN 2230 - 8407. DOI: 10.7897/2230-8407.04306

Rumsey, L. G. (1994). History of early diet development in fish culture, 1000 B. C. to A. D. 1955. The Progressive Fish Culturist. 56(1): 1 - 6. DOI: 10.1577/1548-8640(1994)056%3C0001:HOEDDI%3E2.3.CO;2

Støttrup, G. J. and McEvoy, A.L. (2003). Live Feeds in Marine Aquaculture. Blackwell Science Ltd, Oxford, UK. URL: https://ceebluenterprises.com/wp-content/uploads/2022/08/Live-Feeds-in-Marine-Aquaculture.pdf

Taher, M. M.; Muhammed, S. J.; Salih, O. A.; Al-Dubakel, A. Y. and Mojer, A. M. (2023). Effect of feeding ratio on the growth of common carp cultivated in earthen ponds. Iraqi J. Aquacult. 20(2): 193 - 210. URL: https://iasj.rdd.edu. iq/journals/uploads/2025/01/01/861564d3e72991ece890d89d49b67017.pdf DOI:10.58629/ijaq.v20i2.486

Ukwe, O. I. K.; Edun, O. M and Akinrotimi, O. A. (2018) Comparative Growth Performance and Survival of African Catfish (Clarias gariepinus) Fed with Artemia and Acartia Live Feeds. J. Aquat. Sci. Mar. Biol. 1(3): 24 – 28. DOI:10.22259/2638-5481.0103003 URL: https://sryahwapublications.com/ article/download/2638-5481.0103003

Viveen, W. J. A. R.; Richter, C. J. J.; van Oordt, P. G. W. J., Janssen, J. A. L. and Huisman, E. A. (1985). Practical manual for the culture of the African catfish (Clarias gariepinus). Directorate Gen. Intl. Coop. Min. Foreign Affairs; Dept. Fish Cult. Fish. Agric., Univ. Wageningen, Res. Grp. Comp. Endocrinology; Dept. Zool. Univ. Utrecht. 127 Pp. URL: https://edepot.wur.nl/409535

Zhang, S. Y.; Li, G.; Wu, H. B.; Liu, X. G.; Yao, Y. H.; Tao, L. and Liu, H. (2011). An integrated recirculating aquaculture system (RAS) for land-based fish farming: The effects on water quality and fish production. Aquacultural Engineering 45: 93 - 102. https://doi.org/10.1016/j.aquaeng.2011.08.001 © Elsevier B.V.